Journal:

Article Title: A cholera toxoid-insulin conjugate as an oral vaccine against spontaneous autoimmune diabetes

doi:

Figure Lengend Snippet: T cells from CTB-insulin-fed NOD mice suppress beta cell infiltration by diabetogenic T cells. Immunofluorescence staining of pancreatic islets from irradiated NOD males 30 days after co-injection of syngeneic Thy-1,2+ T cells from 20-week-old diabetic female mice with Thy-1,1+ T cells from congenic NOD mice that were fed CTB-insulin (A, C) or CTB-ovalbumin (B, D). Immunostainings were performed with monoclonal antibodies to Thy-1,2+ (A, B) and Thy-1,1+ (C, D). Original magnifications ×250.

Article Snippet: The percentage of donor Thy-1,1+ T cells in the spleen, mesenteric, and pancreatic lymph nodes of recipient NOD males was determined 7, 15, and 30 days after cell transfer by cytofluorometric analysis using FITC-labeled rat monoclonal antibodies to Thy-1,1 (clone CL005F; Cedarlane Laboratories) or Thy-1,2 (clone 30H12; Biosys, Compiègne, France) ( 32 ).

Techniques: Immunofluorescence, Staining, Irradiation, Injection

Journal: Molecular Medicine Reports

Article Title: Placenta-derived mesenchymal stem cells ameliorate lipopolysaccharide-induced inflammation in RAW264.7 cells and acute lung injury in rats

doi: 10.3892/mmr.2020.11231

Figure Lengend Snippet: Isolation and identification of rat pMSCs. (A) Primary culture and P2 generation cell morphology (magnification, ×100). (B) The three on the left panels are unstained negative controls of flow cytometry analysis, while the six on the right are the quantitative analysis of CD44, CD90, CD29, CD31, CD45 and CD11b/c. (C) Immunofluorescence staining of pMSCs. (a and b) Control group. (c and d) Experimental group. Non-specific nuclear staining is shown in blue on the left-hand side, and specific CD44 staining is in red on the right-hand side. (D) Multi-lineage differentiation capacity of pMSCs. Osteogenesis differentiation was demonstrated by Alizarin Red S staining (magnification, ×100 and ×200), while adipogenic differentiation was demonstrated by Oil Red O staining (magnification, ×100 and ×200). pMSC, placenta-derived mesenchymal stem cell; PE, phycoerythrin; APC, allophycocyanin.

Article Snippet: The supernatant was discarded, and the pellet was resuspended in DMEM containing 10% FBS and plated in T25 Flasks (Corning, Inc.) in a 5% CO 2 air incubator at 37°C (CB 170; BINDER GmbH). pMSCs were identified by flow cytometry (CytoFLEX; Beckman Coulter, Inc.) and the detection of the following cell surface markers: CD11b/c (OX42, PE; cat. no. 12-0110-80; eBioscience; Thermo Fisher Scientific, Inc.); CD44 (OX-49, FITC; cat. no. MA5-17522; Thermo Fisher Scientific, Inc.); CD45 (OX1, APC; cat. no. 47-0461-80; eBioscience; Thermo Fisher Scientific, Inc.); CD90 (HIS51, FITC; cat. no. 03013-50; BioGems; PeproTech, Inc.); CD29 (HMb1-1, FITC; cat. no. 11-0291-80; eBioscience; Thermo Fisher Scientific, Inc.); CD31 (TLD-3A12, FITC; cat. no. MA5-16952; Thermo Fisher Scientific, Inc.).

Techniques: Isolation, Flow Cytometry, Immunofluorescence, Staining, Control, Derivative Assay

Journal: International Journal of Molecular Sciences

Article Title: The 3D World of Spheroids: Searching for an Optimal Method of Fabricating Pro-Reparative Cardiospheres

doi: 10.3390/ijms262412025

Figure Lengend Snippet: Cell and extracellular matrix (ECM) composition of mCSs formed in ULA U-well plates and PDL-coated plates. ( a , b ): Images of mCSs stained by endothelial cell (EC) marker (CD31, red), progenitor cell markers (c-Kit, red; Sca-1, red; Oct4, red), smooth-muscle cell marker (SMA, green), mesenchymal markers (CD105, red; CD90, green; CD73, green), and ECM proteins (COL I, green; FN, green). Nuclei were stained with DAPI. Yellow arrows indicate positive staining. ( c ) Bar plot of a positive staining cell fraction per nuclei within the mCS slice, n = 3, * p < 0.05. ( d ) Bar plot of fluorescence intensity signal normalized per mCS slice area, n = 3, ** p < 0.01. SMA—smooth muscle actin, COL I—collagen type I, FN—fibronectin.

Article Snippet: The EDCs were trypsinized, washed in ice-cold FASC buffer (5% FBS, 0.1% sodium azide in Ca 2+ , Mg 2+ -free DPBS), and incubated with primary antibodies to CD105 (#120404, Biolegend, San Diego, CA, USA), Sca-1 conjugated with PE/Cy7 (E-AB-F1191UH, Elabscience, Wuhan, China), CD90 conjugated with FITC (E-AB-F1283UC, Elabscience), CD31 (#553370; BD, San Diego, CA, USA), and CD45 conjugated with PE (E-AB-F1136UD, Elabscience) or with isotype IgGs for 30 min on ice.

Techniques: Staining, Marker, Fluorescence

Journal: Inflammopharmacology

Article Title: Combined effect of pantoprazole and mesenchymal stem cells on experimentally induced gastric ulcer: implication of oxidative stress, inflammation and apoptosis pathways

doi: 10.1007/s10787-024-01469-0

Figure Lengend Snippet: Flow cytometry characterization of ADSCs showing positive expression of MSCs-specific surface markers (CD90, CD105) and negative expression of hematopoietic-specific marker (CD34)

Article Snippet: After that, they were analyzed for surface markers expression using anti-rat fluorescein isothiocyanate (FITC)-labeled CD90 antibody (R&D Systems, UK), anti-rat FITC-conjugated CD105 antibody (MiltenyiBiotec, Germany), anti-rat FITC-labeled CD34 antibody (Beckman Coulter Co., USA).

Techniques: Flow Cytometry, Expressing, Marker